Fig. 10
Knockdown CLSPN suppressed LUAD cells growth in vivo. A A549 cells or PC9 cells with different CLSPN expression levels were subcutaneously inoculated in the right flank of BALB/c mice (5 × 106 cells/mouse; n = 5 in each group), and then the tumor volume was calculated using the following formula: V (mm3) = (L × W.2) × 0.5 (L: tumor length, W: width). The mice were sacrificed at day 21 after subcutaneous implantation. The formation of tumor masses was presented. B The histogram displayed the tumor weight in different groups. C Tumor size was measured every 3 days until day 21 (Data were represented as the mean ± SD). D Immunohistochemistry staining for Claspin, CCNA2, CCNB1, CDK1, CDK2 and Ki67 in subcutaneous tumors (Scare bar = 50 μm). ***: P < 0.001