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Fig. 6 | Biological Procedures Online

Fig. 6

From: Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3

Fig. 6

CSNK1D activates the Wnt/β-catenin signaling pathway by interacting with DVL3. A CSNK1D interactors were predicted by GENEMANIA database. B DVL3 expression was detected with silencing or overexpressing CSNK1D. (C) Key proteins of Wnt/β-catenin pathway was detected with CSNK1D overexpression and DVL3 silencing. D The interaction between CSNK1D and DVL3 were determined by Co-IP assay in CSNK1D-overexpressed HepG2 cells. E CSNK1D and DVL3 were mainly co-localized in cell nucleus as determined by immunofluorescence. F, G The half-life of DVL3 protein in NC and CSNK1D -overexpressing HepG2 cells was determined by using CHX (100 μg/ml) at indicated time points. H CCK8 assay of HepG2 cells in each group. I, J The colony formation of HepG2 cells in each group. K, L Sphere formation assays of HepG2 cells in each group. M, N Transwell assay of HepG2 cells in each group. O, P The wound healing assay of HepG2 cells in each group

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Biological Procedures Online

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