Table 1 Primers and sequences used to construct the pCFD4d gRNA plasmid to create the genetically modified flies
From: Efficient In Vivo Introduction of Point Mutations Using ssODN and a Co-CRISPR Approach
Name | Sequence (5′ to 3′) |
|---|---|
pCFD4d-tin_sgRNA_F | TATATAGGAAAGATATCCGGGTGAACTTCGgagctgacaaattgcagacaGTTTTAGAGCTAGAAATAGCAAG |
pCFD4d-w_sgRNA_R (tin) | ATTTTAACTTGCTATTTCTAGCTCTAAAACccaaagagcaggaatggtatcCGACGTTAAATTGAAAATAGGTC |
pCFD4d-w_sgRNA_F (Nup107) | TATATAGGAAAGATATCCGGGTGAACTTCGataccattcctgctctttggGTTTTAGAGCTAGAAATAGCAAG |
pCFD4d-Nup107_sgRNA_R | ATTTTAACTTGCTATTTCTAGCTCTAAAACtcagatgggcccagagcaaacCGACGTTAAATTGAAAATAGGTC |
pCFD4d_sequencing_primer | GACACAGCGCGTACGTCCTTCG |
coffee_seq_primer | TATCAACGGAGCCATCTTCCTCTTC |