Fig. 3
From: Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells
Co-localization of EVs stained with TSG101 and calnexin with PKH67. Confocal imaging of PKH67 (green) dye stained the EVs that were immune-labeled using TSG 101 antibody (red). The PKH67 dye (green) got co-localized with TSG101 and yielded yellow signal (merge) (a). Analyses of the percentage of fluorescent signal observed from red (immuno-labeled only), green (PKH67 dye only) and yellow (co-localization of both) channel (b). PKH67 dye retained fluorescent signal whereas calnexin staining was negative in case of dual labeled EVs (c). Confocal imaging of EVs treated with secondary antibodies and PKH67 dye (d and e)