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Table 1 Results from the first sequencing run

From: Modified SureSelectQXT Target Enrichment Protocol for Illumina Multiplexed Sequencing of FFPE Samples

Sample

DIN

#Pre-hyb Cycles

Hyb Input (ng)

#Sequences

Aligned

On target

No PCR Dup

Final

MedianDepth (per million reads)

NA12878

9.3

8

750

3,387,238

99.37%

75.07%

48.16%

48.14%

113.07

NA12891

9.1

8

750

3,188,127

99.48%

72.49%

49.56%

49.54%

118.25

NA12892

9.1

8

750

3,115,861

98.88%

73.80%

50.41%

50.40%

119.07

NT1

6.4

8

401

11,305,259

99.72%

71.49%

31.15%

31.13%

63.42

T1

2.7

10

362.5

2,866,206

99.37%

72.20%

35.17%

35.13%

66.29

T2

2.2

10

11,9

2,813,675

97.65%

81.07%

4.45%

4.40%

4.98

T3

3

10

87

2,730,937

99.39%

77.30%

13.86%

13.84%

25.63

T4

2

10

12

2,347,778

97.87%

71.73%

5.09%

5.05%

7.24

T5

3.6

10

32.4

3,077,225

99.35%

79.63%

8.45%

8.42%

13.65

NA12892

9.1

10

156

2,983,904

98.63%

77.31%

49.21%

49.19%

106.24

  1. From left to right: sample name, DIN value for input DNA, number of cycles in the pre-hyb PCR, total amount of digested and adaptor-tagged library used in the hybridization step, number of sequences obtained, percentage of sequences aligned to the human reference genome, percentage of sequences on target, percentage of sequences after PCR duplicates removal, percentage of sequences after low-quality-mapped-reads removal, median of the depth in the target regions obtained per million reads (normalized for a more realistic comparison)