Table 1 Recipes for Buffers used for the Protocol
Components | Volume |
|---|---|
50X TAE Buffer (500 ml) | |
Tris Buffer GR | 121 g |
EDTA Stock (0.5 M) | 50 ml |
Glacial Acetic Acid | 28.55 ml |
5× TBE Buffer (1 l, pH 8.3) | |
Tris Buffer GR | 54 g |
Boric Acid | 27.5 g |
EDTA Stock (0.5 M) | 20 ml |
30% Acrylamide (25 ml) | |
Acrylamide | 7.3 g |
Bis-acrylamide | 0.2 g |
8% Polyacrylamide Gel | |
30% Acrylamide | 2.620 ml |
5x TBE Buffer | 1.97 ml |
Autoclaved Milli Q water | 5.24 ml |
10% Ammonium Persulfate | 163 μl |
TEMED | 8 μl |
Total | 10 ml |
EtBr (for staining) | 0.5 μg/ml in 1X TBE |
10X TE Buffer (pH 8.0) | |
Tris Buffer | 1 ml (Stock 1 M; Final concentration 100 mM) |
EDTA | 200 μl (Stock 1 M; Final concentration 10 mM) |
Autoclaved Milli Q water | 8.8 ml |