Fig. 3

Developmental processes after embryo transfer of SV40LT-mediated immortalized canine fibroblast cells. a Localization of canine cloned embryos at different stages of development in the reproductive tracts. B, blastocyst; M, morula; n, total number of embryos recovered from each column or row. b Ultrasonographic image of thickened myometrium. A yellow dotted circle indicates well-demarcated uterine wall next to urinary bladder. c Retrieved female reproductive tract. A yellow dotted circle represents implantation scar and bisect uterus. d Histology of implantation site. Scale bar indicates 200 μm. e Multiple scattered hemosiderin spot. Embryos were recovered from surrogates after embryo transfer on day 8. Scale bar indicates 100 μm