Fig. 3

5mC and 5hmC immunolabelling in the embryo gonads at 14dpc, 15dpc and 19dpc. 5mC was not detected in PGC (arrows) at 14dpc (a). At 15dpc (b) 5mC was detected in most PGC (arrows), although very few PGC were still negative (arrowhead). At 19dpc 5mC was detected in all gonocytes (arrows) (c). The labelling of 5hmC was restricted to small nuclear areas at 14dpc (d) and 15dpc (e) PGC (arrowheads). 5hmC-negative PGC were also observed (arrows). The 5hmC-positive areas were more abundant at 14dpc (d) than at 15dpc (e). At 19dpc 5hmC was not detected in gonocytes (arrows) (f)