Fig. 1

Time-dependent Change in the Expression of Endothelial and Smooth Muscle Markers in HMPAECs and HCAECs. a Representative Western blot image comparing changes in the expression of the endothelial marker CD31 and the smooth muscle markers α-SMA and SM22-α in HMPAECs and HCAECs with time in culture. b Quantification of endothelial and smooth muscle markers expression by densitometry analysis of western blots. Results were normalized to α-tubulin (loading control) and expressed as fold-change relative to baseline (day 5 versus day 2 after plating). CD31 (HMPAECs, n = 4; HCAECs, n = 3, non-significant); α-SMA (n = 3, non-significant), SM22-α (n = 3, non-significant)