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Fig. 4 | Biological Procedures Online

Fig. 4

From: A Modified Coupled Spectrophotometric Method to Detect 2-5 Oligoadenylate Synthetase Activity in Prostate Cell Lines

Fig. 4

Enzyme activity in prostate cancer cell line. OAS enzyme assay measurement with pyrophosphate assay in lysates purified from 293T, RwPE1, LNCaP, DU145 and control which is lysate from DU145 cell purified with unactivated sepharose beads with no poly I:C. Shift in absorbance at 360 nm after completion of reaction post addition of PNP and inorganic phosphatase. Assay was done thrice in triplicates. Statistical significance is shown as *** for p < 0.005 and * for p < 0.05

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