Fig. 2

OAS1 protein expression in crude and purified lysates of prostate cancer cell line. OAS1 p46 isoform expression in lysates from 293T, RwPE1, DU145 and LNCaP cell line. a OAS 1 expression in 20 μg crude lysates (upper panel) and GAPDH (lower panel) was used as a loading control. b OAS 1 expression in lysates purified from 20 μg of crude lysates with activated poly I:C bound sepharose beads (upper panel), GAPDH (middle panel) was used as a loading control and elute-1 collected during washing to remove the unbound OAS enzyme and OAS free cell lysates. DU145 lysates purified with unactivated sepharose beads with no poly I:C bound to it was used as a negative control