Fig. 4
Activation of angiogenic growth factors on CAM by SaOS2 cells. Images of Reverse Transcriptase- PCR products of VEGF165 (a), FGF2 (b) MMP2 (c), MMP9 (d) and NOS (e) from CAM after incubated with 20 μl volumes of 3 × 105, 6 × 105 and 12 × 105 SaOS2 cells along with DMEM alone as negative control for 96 h. Images are the representatives of 3 set of experiments. Graph for the fold change in the mRNA level transcript for VEGF165, FGF2, MMP2, MMP9 and NOS after normalizing with GAPDH OD value of the same. The OD value was measured using Image J software. The fold change in the relative level expression of VEGF165 (graph a), FGF2 (graph b), MMP2 (graph c) and MMP9 (graph d) was increased significantly for both 6×105 and 12 × 105 SaOS2 cells/volume at 48 and 96 h of incubation for NOS (graph e) at 96 h for 12 × 105 SaOS2 cells/volume. Each value is the mean ± SEM, p = < 0.001 and p = 0.001 versus control, n = 3