Fig. 2
a. Quantification of angiogenic response of SaOS2 cells on CAM. Angioquant prune images of CAM incubated with 20 μl volume of 3 × 105, 6 × 105 and 12 × 105 cells along with DMEM alone as negative control at 0 and 96 h of incubation. Images taken at 0 and 96 h of incubation were subsequently analyzed by Angioquant software. All three volumes of SaOS2 cells shows numerous scattered allantoic vessels at the chorioallantoic layer of CAM (d, f, and h) compared to control. Red line indicated the presence of blood vessels around the sponge. Images are representative of 3 set of experiments. b. SaOS2 cells are able to induce the growth and sprouting ability of vessels on CAM. Total length, size and number of junctions of the blood vessels were measured using Angioquant software from the images taken at 0 and 96 h of incubation. Growth of the vessels was confirmed by measuring the length and size of the vessels and sprouting ability by means of measuring the number of vessels junctions. All three volumes of cells shows comparatively significant increase in the length (Graph A), size (Graph B) and number of vessels junction (Graph C) at 96 h of incubation. The value at 0 h considers as one for all cases. Experiments were performed in triplicate and data presented as mean ± SEM, p = < 0.001, p = 0.001 and p = 0.010 versus control, n = 3