Fig. 1
a . SaOS2 cells induces new blood vessel growth on CAM vascular bed. Images of late CAM incubated with SaOS2 cell line for 96 h. Gelatin sponges loaded with 20 μl volume of 3 × 105, 6 × 105 and 12 × 105 cells along with DMEM alone as negative control were placed on CAM and vascularization was analyzed visually from the images taken at 0, 48 and 96 h of incubation. All three volumes of SaOS2 cells shows the presence of vertically growing blood vessels around the sponge at 48 and 96 h of incubation. CAM incubated with 6×105 and 12 × 105 cells/volume shows numerous scattered and spoke wheel pattern of allantoic vessel growth around the sponge which is indicated by the reddishness around the sponge (h, i, k and l). Images are taken using Cannon digital camera at 4× magnification and are representative of 3 set of experiments. Arrow indicates the presence of blood vessels and bar is 10 μm. b. Growth of blood vessels around the sponge. Angiogenic ability of SaOS2 cells on CAM vascular bed conformed by counting the number of newly formed vessels around the sponge. CAM incubated with 6×105 and 12 × 105 cells/volume shows significant increase in the number of newly formed allantoic vessels around the sponge at 96 h of incubation than control. Experiments were performed in triplicate and data presented as mean ± SEM, p = < 0.001 and p = 0.005 versus control, n = 3