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Table 4 qPCR conditions 1

From: A convenient, optimized pipeline for isolation, fluorescence microscopy and molecular analysis of live single cells

Cycle #

Temperature

Time

1

95°C

30 seconds

2-42

95°C

5 seconds

 

60°C (Collect Data)

30 seconds

43

Melt Curve Analysis

Instrument Dependent

  1. 1Thermal cycling conditions may vary depending on reagent manufacturer.
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Biological Procedures Online

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