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Table 2 RT-qPCR primers

From: A convenient, optimized pipeline for isolation, fluorescence microscopy and molecular analysis of live single cells

Gene target

Accession #

Forward sequence

Reverse sequence

Amplicon size

Beta-actin (Human)

NM_001101.3

5′-ctggaacggtgaaggtgaca

5′-aagggacttcctgtaacaacgca

140 bp

GFP (TurboGFP)

GU452685.1

5′-aggacagcgtgatcttcacc

5′-cttgaagtgcatgtggctgt

164 bp