Figure 3

Cell lysis procedure. A visual reference for performing cell lysis as described in the step-wise protocol. A) Total medium from target wells are transferred to a PCR tube containing 10 μL RNA lysis buffer and pipetted up and down. B) RNA lysis buffer is added to the target well to the same PCR tube as in A. Procedures B and C are repeated once for a total of 3 transfers from each target well.