Figure 1

Schematic diagram of the recombinant virus AcW-Bt7 construction. (A) The Cry1Ab gene (2.2 kb) was PCR amplified using WG1 and WG2 specific primers introducing PstI and NcoI restriction sites to its 5' and 3' ends, respectively. The digested Cry1Ab-PstI/NcoI fragment was cloned into Pst/NcoI sites of pBlueBacIII transfer vector generating the recombinant plasmid wpBac-Bt7. The features of pBlueBacIII carrying the truncated form of Cry1Ab gene under control of Polyhedrin promoter are shown. (B) Upon co-transfection of the wpBac-Bt7 transfer vector and the linearized Bac-N-Blue DNA into Sf9 cells, homologous recombination between ORF1629 sequences and lacZ sequences are occurred generating the recombinant virus AcW-Bt7.