Figure 3

Overview of experimental system. An ex vivo perfusion fluid-flow setup is shown to maintain a healthy functional tissue for the duration of experiment. a. The setup includes a computer with MetaMorph software (MM), syringe pump (SP), perfusion syringe (PS), perfusion apparatus (PA), collection pump (CP), collection flask (CF), incubation chamber (IC), gas tank (GT). b. Not visible in picture is a temperature controller (TC), humidity controller (HC), and computer monitor. Artery segment has a baseline fluorescent after loaded with calcium indicator. c. Brightfield image of the artery segment is visualized. As depicted by the diagram below, a perfusion needle is cannulated to the artery to deliver fluid-flow.