Figure 2
From: Real-time vascular mechanosensation through ex vivo artery perfusion
Schematic representation of perfusion apparatus. a. An inexpensive apparatus can be made using a 100-mm cell culture dish, a 22-g hypodermic needle, glass capillary tubes, a glass cover slip, silicone tubing, and non-cytotoxic epoxy and silicone glues. b. After cannulation to the apparatus, the vessel is covered with a cover glass. The chamber is placed on the microscope and connected with the rest of perfusion system.