Table 1 RT reaction components
From: Detection and quantification of extracellular microRNAs in murine biofluids
Component | μl per reaction (1X) |
|---|---|
10X miRNA RT buffer | 2 |
MultiScribe RT (50 U/μl) | 1 |
100 mM dNTP mix | 0.15 |
RNase Inhibitor (20 U/μl) | 0.18 |
20X miRNA RT primer - assay A | 1 |
20X miRNA RT primer - assay B | 1 |
20X miRNA RT primer - assay C | 1 |
20X miRNA RT primer - assay D | 1 |
Water | 7.67 |
(Total) | (15) |