Figure 1

Viability, recovery and the expression of CD45, CD11c and CD11b in mouse oral intra-epithelial and lamina propria leukocytes (MOIL) isolated from the oral tissues of mice. Flow cytometric histograms showing propidium iodide (PI) uptake by the dead cells (A), or dot plots showing conventional leukocyte gating in our flow cytometry analyses (B), in crude cell preparations (top) and those isolated by percoll separation (bottom). (C) The percentage of viable cells (PI negative), as assessed by flow cytometry as in (A). (D), MOIL cell recovery (cell count/1 mouse) from various strains of mice, as enumerated using hemocytometer. (E) Flow cytometric contour plots showing CD45.2, CD11b and CD11c expression, which are the hematopoietic, monocytic, and dendritic cell markers, respectively. Percentages of marker expressing MOIL (pooled from 2 mice) are shown in the gates. All these data represent at least three to five independent experiments using at least 10- 15 mice. P values were calculated by Students ‘t’ test in Microsoft Excel software using two tailed distribution and two-sample equal variance parameters.