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Table 10 Components for colony PCR

From: A do-it-yourself protocol for simple transcription activator-like effector assembly

Component

Amount (μL)

dNTP

0.2

Colony-PCR primer mix

0.2

Taq Polymerase (5 U μL-1)

0.1

Taq Polymerase buffer, 10x

2

DNA-grade H2O

16.5

total

19