Table 1 Volume for preparation of chitosan membranes dependnt on the type of culture plate
From: Solution casting of chitosan membranes for in vitro evaluation of bioactivity
| Â | Chitosan solution | NaOH (neutralization) | 70% ethanol (sterilization) | Fibronectin solution | Cell culture media | Alizarin Red Stain | Crystal Violet Stain |
|---|---|---|---|---|---|---|---|
6-well plate | 1Â ml | 2Â ml | 2Â ml | 2Â ml | 3Â ml | 2Â ml | 2Â ml |
12-well plate | 500 μl | 1 ml | 1 ml | 1 ml | 1.5 ml | 1 ml | 1 ml |
24-well plate | 250 μl | 500 μl | 500 μl | 500 μl | 1 ml | 1 ml | 1 ml |
96-well plate | 100 μl | 200 μl | 200 μl | 200 μl | 100 μl | 100 μl | 100 μl |
2-well slide chamber | 500 μl | 1 ml | 1 ml | 1 ml | 2 ml | 1 ml | 1 ml |