Figure 1From: A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphateMg2+ concentration dependence of 45Ca2+ uptake in ER microsomes isolated from male rat brain. Net 45Ca2+ accumulation was measured by liquid scintillation counting after the uptake reaction was allowed to proceed for 60 minutes at 37°C in buffer containing 300 nM free Ca2+ and 2 mM ATP (see materials and methods). Data are means ± SEM, n = 3.Back to article page