Figure 5From: Critical Appraisal of the MTT Assay in the Presence of Rottlerin and Uncouplers Effect of Rottlerin and FCCP on MTT assay. Cells were treated for 30 min with 5 and 20 μM Rottlerin and FCCP (R5, R20 and F5, F20, respectively) or vehicle alone (DMSO and ethanol, respectively) and MTT reduction was evaluated as described in "Materials and methods". Values are the average of three separate experiments in quadruplicate and are expressed as mean ± SD. *p < 0.05.Back to article page